Abstract

Hippocampal activity influences neurogenesis in the adult dentate gyrus; however, little is known about the involvement of the hippocampal circuitry in this process. In the subgranular zone of the adult dentate gyrus, neurogenesis involves a series of differentiation steps from radial glia-like stem/progenitor (type-1) cells, to transiently amplifying neuronal progenitor (type-2) cells, to postmitotic neurons. In this study, we conducted GFP-targeted recordings of progenitor cells in fresh hippocampal slices from nestin-GFP mice and found that neuronal progenitor (type-2) cells receive active direct neural inputs from the hippocampal circuitry. This input was GABAergic but not glutamatergic. The GABAergic inputs depolarized type-2 cells because of their elevated [Cl(-)](i). This excitation initiated an increase of [Ca(2+)](i) and the expression of NeuroD. A BrdU-pulse labeling study with GABA(A)-R agonists demonstrated the promotion of neuronal differentiation via this GABAergic excitation. Thus, it appears that GABAergic inputs to hippocampal progenitor cells promote activity-dependent neuronal differentiation.

Keywords

GABAergicNeuroscienceHippocampal formationProgenitor cellProgenitorBiologyHippocampusPsychologyCell biologyStem cellInhibitory postsynaptic potential

MeSH Terms

4-AminopyridineAnimalsBasic Helix-Loop-Helix Transcription FactorsBicucullineBromodeoxyuridineCalbindinsCalciumCell CountCell DifferentiationDrug InteractionsElectric StimulationExcitatory Amino Acid AgonistsGABA AntagonistsGlycineGreen Fluorescent ProteinsHippocampusHistoryAncientImmunohistochemistryIn Vitro TechniquesIntermediate Filament ProteinsKi-67 AntigenMembrane PotentialsMiceMiceTransgenicMicroscopyImmunoelectronN-MethylaspartateNerve Tissue ProteinsNestinNeural Cell Adhesion Molecule L1NeuronsNickelPatch-Clamp TechniquesPhosphopyruvate HydratasePotassium Channel BlockersS100 Calcium Binding Protein GSialic AcidsSodium-Potassium-Chloride SymportersSolute Carrier Family 12Member 2Stem CellsTetrodotoxinVesicular Inhibitory Amino Acid Transport Proteinsalpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acidgamma-Aminobutyric Acid

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Publication Info

Year
2005
Type
article
Volume
47
Issue
6
Pages
803-815
Citations
723
Access
Closed

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Cite This

Yusuke Tozuka, Satoshi Fukuda, Takashi Namba et al. (2005). GABAergic Excitation Promotes Neuronal Differentiation in Adult Hippocampal Progenitor Cells. Neuron , 47 (6) , 803-815. https://doi.org/10.1016/j.neuron.2005.08.023

Identifiers

DOI
10.1016/j.neuron.2005.08.023
PMID
16157276

Data Quality

Data completeness: 86%