From Marrow to Brain: Expression of Neuronal Phenotypes in Adult Mice

Abstract

After intravascular delivery of genetically marked adult mouse bone marrow into lethally irradiated normal adult hosts, donor-derived cells expressing neuronal proteins (neuronal phenotypes) developed in the central nervous system. Flow cytometry revealed a population of donor-derived cells in the brain with characteristics distinct from bone marrow. Confocal microscopy of individual cells showed that hundreds of marrow-derived cells in brain sections expressed gene products typical of neurons (NeuN, 200-kilodalton neurofilament, and class III β-tubulin) and were able to activate the transcription factor cAMP response element–binding protein (CREB). The generation of neuronal phenotypes in the adult brain 1 to 6 months after an adult bone marrow transplant demonstrates a remarkable plasticity of adult tissues with potential clinical applications.

Keywords

NeuNPhenotypeBiologyBone marrowPathologyHaematopoiesisCell biologyFlow cytometryCREBNeurofilamentTranscription factorImmunologyStem cellGeneImmunohistochemistryMedicineGenetics

Affiliated Institutions

Stanford University US

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Publication Info

Year: 2000
Type: article
Volume: 290
Issue: 5497
Pages: 1775-1779
Citations: 1682
Access: Closed

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APA Style

                            
                                
                                    Timothy R. Brazelton, 
                                
                                    Fábio Rossi, 
                                
                                    Gilmor I. Keshet
                                
                                et al.
                            
                            (2000). 
                            From Marrow to Brain: Expression of Neuronal Phenotypes in Adult Mice. 
                            Science
                            , 290
                            (5497)
                            , 1775-1779.
                            https://doi.org/10.1126/science.290.5497.1775
                        

Identifiers

DOI: 10.1126/science.290.5497.1775