Direct isolation of human central nervous system stem cells

Abstract

Stem cells, which are clonogenic cells with self-renewal and multilineage differentiation properties, have the potential to replace or repair damaged tissue. We have directly isolated clonogenic human central nervous system stem cells (hCNS-SC) from fresh human fetal brain tissue, using antibodies to cell surface markers and fluorescence-activated cell sorting. These hCNS-SC are phenotypically 5F3 (CD133) + , 5E12 + , CD34 − , CD45 − , and CD24 −/lo . Single CD133 + CD34 − CD45 − sorted cells initiated neurosphere cultures, and the progeny of clonogenic cells could differentiate into both neurons and glial cells. Single cells from neurosphere cultures initiated from CD133 + CD34 − CD45 − cells were again replated as single cells and were able to reestablish neurosphere cultures, demonstrating the self-renewal potential of this highly enriched population. Upon transplantation into brains of immunodeficient neonatal mice, the sorted/expanded hCNS-SC showed potent engraftment, proliferation, migration, and neural differentiation.

Keywords

NeurosphereBiologyStem cellClonogenic assayCell sortingCell biologyNeural stem cellCD34Stem cell markerPopulationCellular differentiationMolecular biologyImmunologyCell cultureAdult stem cellFlow cytometryGeneticsMedicine

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Publication Info

Year: 2000
Type: article
Volume: 97
Issue: 26
Pages: 14720-14725
Citations: 1812
Access: Closed

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APA Style

                            
                                
                                    Nobuko Uchida, 
                                
                                    David Buck, 
                                
                                    Dongping He
                                
                                et al.
                            
                            (2000). 
                            Direct isolation of human central nervous system stem cells. 
                            Proceedings of the National Academy of Sciences
                            , 97
                            (26)
                            , 14720-14725.
                            https://doi.org/10.1073/pnas.97.26.14720
                        

Identifiers

DOI: 10.1073/pnas.97.26.14720