RIKEN Center for Brain Science

An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein

We have cloned a gene encoding a fluorescent protein from a stony coral, Trachyphyllia geoffroyi , which emits green, yellow, and red light. The protein, named Kaede, includes a...

Regulated Fast Nucleocytoplasmic Shuttling Observed by Reversible Protein Highlighting

The observation of the regulation of fast protein dynamics in a cellular context requires the development of reliable technologies. Here, a signal regulation cascade reliant on ...

Monitoring protein conformations and interactions by fluorescence resonance energy transfer between mutants of green fluorescent protein

Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer

GFP (green fluorescent protein)-based FRET (fluorescence resonance energy transfer) technology has facilitated the exploration of the spatio-temporal patterns of cellular signal...

Semi‐rational engineering of a coral fluorescent protein into an efficient highlighter

Photo-Induced Peptide Cleavage in the Green-to-Red Conversion of a Fluorescent Protein

Self-whitening algorithms for adaptive equalization and deconvolution

In equalization and deconvolution tasks, the correlated nature of the input signal slows the convergence speeds of stochastic gradient adaptive filters. Prewhitening techniques ...