Keywords
FluorescenceFörster resonance energy transferGreen fluorescent proteinFluorescent proteinMutantChemistryEnergy transferBiophysicsResonance (particle physics)Bimolecular fluorescence complementationFluorescence in the life sciencesPhotochemistryNuclear magnetic resonanceChemical physicsBiochemistryBiologyPhysicsAtomic physicsOpticsGene
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Probing the interaction between two single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor.
We extend the sensitivity of fluorescence resonance energy transfer (FRET) to the single molecule level by measuring energy transfer between a single donor fluorophore and a sin...
Publication Info
- Year
- 2000
- Type
- article
- Volume
- 327
- Pages
- 472-500
- Citations
- 406
- Access
- Closed
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Atsushi Miyawaki,
Roger Y. Tsien
(2000).
Monitoring protein conformations and interactions by fluorescence resonance energy transfer between mutants of green fluorescent protein.
Methods in enzymology on CD-ROM/Methods in enzymology
, 327
, 472-500.
https://doi.org/10.1016/s0076-6879(00)27297-2
Identifiers
- DOI
- 10.1016/s0076-6879(00)27297-2