Abstract
The observation of the regulation of fast protein dynamics in a cellular context requires the development of reliable technologies. Here, a signal regulation cascade reliant on the stimulus-dependent acceleration of the bidirectional flow of mitogen-activated protein kinase (extracellular signal-regulated kinase) across the nuclear envelope was visualized by reversible protein highlighting. Light-induced conversion between the bright and dark states of a monomeric fluorescent protein engineered from a novel coral protein was employed. Because of its photochromic properties, the protein could be highlighted, erased, and highlighted again in a nondestructive manner, allowing direct observation of regulated fast nucleocytoplasmic shuttling of key signaling molecules.
Keywords
Cell biologyProtein kinase ABiophysicsChemistryExtracellularKinaseNuclear localization sequenceProtein dynamicsBiologyProtein structureBiochemistryCytoplasm
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Publication Info
- Year
- 2004
- Type
- article
- Volume
- 306
- Issue
- 5700
- Pages
- 1370-1373
- Citations
- 822
- Access
- Closed
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Cite This
Ryoko Ando,
Hideaki Mizuno,
Atsushi Miyawaki
(2004).
Regulated Fast Nucleocytoplasmic Shuttling Observed by Reversible Protein Highlighting.
Science
, 306
(5700)
, 1370-1373.
https://doi.org/10.1126/science.1102506
Identifiers
- DOI
- 10.1126/science.1102506