Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing

Keywords

Amplicon sequencingIllumina dye sequencingComputational biologyAmpliconDeep sequencingBiologyDNA sequencingQuality (philosophy)Computer scienceGeneticsGeneGenomePolymerase chain reaction

Affiliated Institutions

Related Publications

Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms

J. Gregory Caporaso , Christian L. Lauber , William A. Walters +11 more

Abstract DNA sequencing continues to decrease in cost with the Illumina HiSeq2000 generating up to 600 Gb of paired-end 100 base reads in a ten-day run. Here we present a protoc...

2012 The ISME Journal 8644 citations

Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform

James J. Kozich , Sarah L. Westcott , Nielson T. Baxter +2 more

ABSTRACT Rapid advances in sequencing technology have changed the experimental landscape of microbial ecology. In the last 10 years, the field has moved from sequencing hundreds...

2013 Applied and Environmental Microbiology 7128 citations

Reducing the Effects of PCR Amplification and Sequencing Artifacts on 16S rRNA-Based Studies

Patrick D. Schloss , Dirk Gevers , Sarah L. Westcott

The advent of next generation sequencing has coincided with a growth in interest in using these approaches to better understand the role of the structure and function of the mic...

2011 PLoS ONE 2094 citations

Comparison of Illumina paired-end and single-direction sequencing for microbial 16S rRNA gene amplicon surveys

Jeffrey J. Werner , Dennis W. Zhou , J. Gregory Caporaso +2 more

Comparison of Illumina paired-end and single-direction sequencing for microbial 16S rRNA gene amplicon surveys

2011 The ISME Journal 126 citations

Short pyrosequencing reads suffice for accurate microbial community analysis

Zhirong Liu , Catherine Lozupone , Micah Hamady +2 more

Pyrosequencing technology allows us to characterize microbial communities using 16S ribosomal RNA (rRNA) sequences orders of magnitude faster and more cheaply than has previousl...

2007 Nucleic Acids Research 671 citations

Publication Info

Year: 2012
Type: article
Volume: 10
Issue: 1
Pages: 57-59
Citations: 4162
Access: Closed

External Links

View on DOI.org

Social Impact

Altmetric

Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing

PlumX Metrics

Social media, news, blog, policy document mentions

Citation Metrics

4162

OpenAlex

Cite This

APA Style

                            
                                
                                    Nicholas A. Bokulich, 
                                
                                    Sathish Subramanian, 
                                
                                    Jeremiah J. Faith
                                
                                et al.
                            
                            (2012). 
                            Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing. 
                            Nature Methods
                            , 10
                            (1)
                            , 57-59.
                            https://doi.org/10.1038/nmeth.2276
                        

Identifiers

DOI: 10.1038/nmeth.2276