Abstract

Abstract DNA sequencing continues to decrease in cost with the Illumina HiSeq2000 generating up to 600 Gb of paired-end 100 base reads in a ten-day run. Here we present a protocol for community amplicon sequencing on the HiSeq2000 and MiSeq Illumina platforms, and apply that protocol to sequence 24 microbial communities from host-associated and free-living environments. A critical question as more sequencing platforms become available is whether biological conclusions derived on one platform are consistent with what would be derived on a different platform. We show that the protocol developed for these instruments successfully recaptures known biological results, and additionally that biological conclusions are consistent across sequencing platforms (the HiSeq2000 versus the MiSeq) and across the sequenced regions of amplicons.

Keywords

BiologyAmplicon sequencingAmpliconDNA sequencingComputational biologyIllumina dye sequencingMetagenomicsDeep sequencingGeneticsBioinformaticsGeneGenomePolymerase chain reaction

Affiliated Institutions

Related Publications

Publication Info

Year
2012
Type
article
Volume
6
Issue
8
Pages
1621-1624
Citations
8644
Access
Closed

External Links

View on DOI.org

Social Impact

Altmetric
PlumX Metrics

Social media, news, blog, policy document mentions

Citation Metrics

8644
OpenAlex

Cite This

J. Gregory Caporaso, Christian L. Lauber, William A. Walters et al. (2012). Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms. The ISME Journal , 6 (8) , 1621-1624. https://doi.org/10.1038/ismej.2012.8

Identifiers

DOI
10.1038/ismej.2012.8