MicroRNA Expression in Zebrafish Embryonic Development

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs, about 21 nucleotides in length, that can regulate gene expression by base-pairing to partially complementary mRNAs. Regulation by miRNAs can play essential roles in embryonic development. We determined the temporal and spatial expression patterns of 115 conserved vertebrate miRNAs in zebrafish embryos by microarrays and by in situ hybridizations, using locked-nucleic acid–modified oligonucleotide probes. Most miRNAs were expressed in a highly tissue-specific manner during segmentation and later stages, but not early in development, which suggests that their role is not in tissue fate establishment but in differentiation or maintenance of tissue identity.

Keywords

ZebrafishBiologymicroRNAIn situ hybridizationGene expressionRegulation of gene expressionVertebrateDNA microarrayCell biologyOligonucleotideGeneGeneticsEmbryonic stem cellRNAComputational biology

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Publication Info

Year: 2005
Type: article
Volume: 309
Issue: 5732
Pages: 310-311
Citations: 1541
Access: Closed

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APA Style

                            
                                
                                    Erno Wienholds, 
                                
                                    Wigard P. Kloosterman, 
                                
                                    Eric A. Miska
                                
                                et al.
                            
                            (2005). 
                            MicroRNA Expression in Zebrafish Embryonic Development. 
                            Science
                            , 309
                            (5732)
                            , 310-311.
                            https://doi.org/10.1126/science.1114519
                        

Identifiers

DOI: 10.1126/science.1114519