Abstract

MicroRNAs (miRNAs) are a class of small noncoding RNA genes recently found to be abnormally expressed in several types of cancer. Here, we describe a recently developed methodology for miRNA gene expression profiling based on the development of a microchip containing oligonucleotides corresponding to 245 miRNAs from human and mouse genomes. We used these microarrays to obtain highly reproducible results that revealed tissue-specific miRNA expression signatures, data that were confirmed by assessment of expression by Northern blots, real-time RT-PCR, and literature search. The microchip oligolibrary can be expanded to include an increasing number of miRNAs discovered in various species and is useful for the analysis of normal and disease states.

Keywords

microRNABiologyOligonucleotideDNA microarrayComputational biologyGene expression profilingGenomeGeneHuman genomeGene expressionRNAGeneticsMolecular biology

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Publication Info

Year
2004
Type
article
Volume
101
Issue
26
Pages
9740-9744
Citations
942
Access
Closed

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Cite This

Chang‐Gong Liu, George A. Calin, Brian Meloon et al. (2004). An oligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues. Proceedings of the National Academy of Sciences , 101 (26) , 9740-9744. https://doi.org/10.1073/pnas.0403293101

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DOI
10.1073/pnas.0403293101