Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia

Abstract

Micro-RNAs ( miR genes) are a large family of highly conserved noncoding genes thought to be involved in temporal and tissue-specific gene regulation. MiRs are transcribed as short hairpin precursors (≈70 nt) and are processed into active 21- to 22-nt RNAs by Dicer, a ribonuclease that recognizes target mRNAs via base-pairing interactions. Here we show that miR15 and miR16 are located at chromosome 13q14, a region deleted in more than half of B cell chronic lymphocytic leukemias (B-CLL). Detailed deletion and expression analysis shows that miR15 and miR16 are located within a 30-kb region of loss in CLL, and that both genes are deleted or down-regulated in the majority (≈68%) of CLL cases.

Keywords

DicerBiologyGeneChronic lymphocytic leukemiamicroRNAGeneticsDroshaRibonuclease IIIRNARibonucleaseGene expressionMolecular biologyLeukemiaRNA interference

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Publication Info

Year: 2002
Type: article
Volume: 99
Issue: 24
Pages: 15524-15529
Citations: 4969
Access: Closed

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APA Style

                            
                                
                                    George A. Calin, 
                                
                                    Calin Dan Dumitru, 
                                
                                    Masayoshi Shimizu
                                
                                et al.
                            
                            (2002). 
                            Frequent deletions and down-regulation of micro- RNA genes <i>miR15</i> and <i>miR16</i> at 13q14 in chronic lymphocytic leukemia. 
                            Proceedings of the National Academy of Sciences
                            , 99
                            (24)
                            , 15524-15529.
                            https://doi.org/10.1073/pnas.242606799
                        

Identifiers

DOI: 10.1073/pnas.242606799