Abstract

Abstract Supernatants from some mouse helper T cell (TH) lines contain an activity that can enhance IgE production by lipopolysaccharide (LPS)-stimulated B cells by at least two orders of magnitude. During purification, this activity could not be resolved from B cell stimulatory factor-1 (BSF-1). Highly purified BSF-1 from a different source, the T lymphoma cell line EL-4, enhanced IgE production to the same extent as TH supernatants, which suggests that BSF-1 is responsible for this increase in IgE production. Monoclonal antibody to BSF-1 totally inhibits the IgE-enhancing activity of a TH supernatant, lending further support to this conclusion. The effects of BSF-1 on LPS-stimulated B cells are specific for IgE and, as previously reported, IgG1 and IgG3, because the levels of IgM, IgG2a, IgG2b, and IgA in the cultures change relatively little when BSF-1 is added.

Keywords

Immunoglobulin ELipopolysaccharideB cellCell cultureMonoclonal antibodyCD23Molecular biologyBiologyAntibodyChemistryImmunology

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Publication Info

Year
1986
Type
article
Volume
136
Issue
12
Pages
4538-4541
Citations
796
Access
Closed

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Cite This

Robert L. Coffman, J Ohara, M W Bond et al. (1986). B cell stimulatory factor-1 enhances the IgE response of lipopolysaccharide-activated B cells.. The Journal of Immunology , 136 (12) , 4538-4541. https://doi.org/10.4049/jimmunol.136.12.4538

Identifiers

DOI
10.4049/jimmunol.136.12.4538