Abstract
The addition of TGF-beta to cultures of LPS-stimulated murine B cells causes a approximately 10-fold enhancement of IgA production, yet causes a 10-fold decrease in total Ig production. IL-2 and, to a lesser extent, IL-5 synergize with TGF-beta to further enhance IgA production and partially reverse the inhibition of total Ig production. IgA constitutes only approximately 0.1% of total Ig in LPS-stimulated cultures, but that percentage rises to 15-25% in cultures to which TGF-beta and IL-2 are added. TGF-beta induces a substantial increase in IgA production from sIgA- B cells but inhibits IgA production by sIgA+ cells. This finding suggests that TGF-beta acts as an isotype-specific switch factor for IgA.
Keywords
IsotypeLipopolysaccharideTransforming growth factor betaImmunoglobulin ATransforming growth factorBiologyAntibodyImmunologyMolecular biologyImmunoglobulin GEndocrinologyMonoclonal antibody
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Publication Info
- Year
- 1989
- Type
- article
- Volume
- 170
- Issue
- 3
- Pages
- 1039-1044
- Citations
- 576
- Access
- Closed
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Cite This
Robert L. Coffman,
Deborah A. Lebman,
B Shrader
(1989).
Transforming growth factor beta specifically enhances IgA production by lipopolysaccharide-stimulated murine B lymphocytes..
The Journal of Experimental Medicine
, 170
(3)
, 1039-1044.
https://doi.org/10.1084/jem.170.3.1039
Identifiers
- DOI
- 10.1084/jem.170.3.1039