Abstract

Abstract IL-4 is able to preferentially enhance murine IgE levels in the supernatant of LPS-stimulated T cell-depleted splenic B cell cultures. Clonal and quantitative analysis of this response revealed that this is due partly to a 14-fold increased IgE precursor frequency and partly to a three-fold increased clone size of IgE-secreting cells. IL-4 increased the precursor frequency and the clone size of IgM-secreting cells not more than twofold. Both the IgM and IgE response in LPS-stimulated B cells were completely inhibited by the addition of anti-IgM mAb (M41) to the cultures, indicating that the IgE-secreting clones developed as subclones from precursors that express IgM. These cells lacked expression of membrane-bound IgE up to day 5 of the culture. Application of feeder cells in these cultures resulted in an increased precursor frequency of IgE-secreting clones among LPS-reactive B cells that is due, partially, to IL-4 produced by the feeder cells.

Keywords

Immunoglobulin Eclone (Java method)BiologyMolecular biologyInterleukin 4AntibodyImmunologyCell cultureB cellCytokineGeneBiochemistry

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Year
1988
Type
article
Volume
141
Issue
3
Pages
749-755
Citations
36
Access
Closed

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H.F.J. Savelkoul, Deborah A. Lebman, R Benner et al. (1988). Increase of precursor frequency and clonal size of murine IgE-secreting cells by IL-4.. The Journal of Immunology , 141 (3) , 749-755. https://doi.org/10.4049/jimmunol.141.3.749

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DOI
10.4049/jimmunol.141.3.749