Keywords
Real-time polymerase chain reactionQuantitative analysis (chemistry)DeltaComputational biologySample (material)BiologyBiological systemComputer scienceMolecular biologyGeneChromatographyChemistryGeneticsPhysics
Affiliated Institutions
Related Publications
Relative expression software tool (REST(C)) for group-wise comparison and statistical analysis of relative expression results in real-time PCR
Real-time reverse transcription followed by polymerase chain reaction (RT-PCR) is the most suitable method for the detection and quantification of mRNA. It offers high sensitivi...
A novel method for real time quantitative RT-PCR.
A novel approach to quantitative reverse transcriptase polymerase chain reaction (QC RT-PCR) using real time detection and the 5' nuclease assay has been developed. Cystic fibro...
Systematic analysis of microRNA expression of RNA extracted from fresh frozen and formalin-fixed paraffin-embedded samples
microRNAs (miRNAs) are noncoding small RNAs that regulate gene expression at the translational level by mainly interacting with 3′ UTRs of their target mRNAs. Archived formalin-...
Real time quantitative PCR.
We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This m...
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
The reverse transcription polymerase chain reaction (RT-PCR) is the most sensitive method for the detection of low-abundance mRNA, often obtained from limited tissue samples. Ho...
Publication Info
- Year
- 2001
- Type
- article
- Volume
- 25
- Issue
- 4
- Pages
- 402-408
- Citations
- 174148
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
174148
OpenAlex
Cite This
Kenneth J. Livak,
Thomas D. Schmittgen
(2001).
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method.
Methods
, 25
(4)
, 402-408.
https://doi.org/10.1006/meth.2001.1262
Identifiers
- DOI
- 10.1006/meth.2001.1262