Real time quantitative PCR. | RDL Research Database

Abstract

We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over contamination and resulting in much faster and higher throughput assays. The real-time PCR method has a very large dynamic range of starting target molecule determination (at least five orders of magnitude). Real-time quantitative PCR is extremely accurate and less labor-intensive than current quantitative PCR methods.

Keywords

Real-time polymerase chain reactionTaqManBiologyComputational biologyDigital polymerase chain reactionQuantitative analysis (chemistry)Primer dimerMolecular biologyPolymerase chain reactionChromatographyGeneGeneticsChemistry

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Publication Info

Year: 1996
Type: article
Volume: 6
Issue: 10
Pages: 986-994
Citations: 6173
Access: Closed

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APA Style

                            
                                
                                    Chris Heid, 
                                
                                    Junko Stevens, 
                                
                                    Kenneth J. Livak
                                
                                et al.
                            
                            (1996). 
                            Real time quantitative PCR.. 
                            Genome Research
                            , 6
                            (10)
                            , 986-994.
                            https://doi.org/10.1101/gr.6.10.986
                        

Identifiers

DOI: 10.1101/gr.6.10.986