Michael W. Pfaffl - Researcher Profile

Publications

4 shown

Quantification of insulin-like growth factor-1 (IGF-1) mRNA: Development and validation of an internally standardised competitive reverse transcription-polymerase chain reaction

Michael W. Pfaffl , Heinrich Meyer , Helga Sauerwein

To investigate the role of local IGF-1 mRNA expression in various tissues, we developed and validated a method which allows for a specific, sensitive and reliable quantification...

2009 Experimental and Clinical Endocrinolo... 41 citations

Relative expression software tool (REST(C)) for group-wise comparison and statistical analysis of relative expression results in real-time PCR

Michael W. Pfaffl

Real-time reverse transcription followed by polymerase chain reaction (RT-PCR) is the most suitable method for the detection and quantification of mRNA. It offers high sensitivi...

2002 Nucleic Acids Research 7261 citations