Howard Cedar

Linking DNA methylation and histone modification: patterns and paradigms

Effect of regional DNA methylation on gene expression.

The effect of DNA methylation on the transcriptional activity of the hamster adenine phosphoribosyltransferase (aprt) and the herpes thymidine kinase (tk) genes has been investi...

Distribution of 5-methylcytosine in chromatin.

The content of 5-methylcytosine in eukaryotic DNA was measured by mass spectrometry. Almost equal amounts of methylated cytosine were found in the DNA of various tissues of the ...

Effect of CpG methylation on<i>Msp</i>I

The restriction enzyme Msp I is inhibited by the presence of a methyl moiety at the external cytosine of the sequence CCGG, but is generally unaffected by methylation at the int...

Spl elements protect a CpG island from de novo methylation

Direct detection of methylated cytosine in DNA by use of the restriction enzyme Mspl

The extent of methylation of the internal C in the sequence CCGG in DNA from various eukaryotic sources has been determined using the restriction enzyme MspI known to be specifi...