Abstract
The restriction enzyme Msp I is inhibited by the presence of a methyl moiety at the external cytosine of the sequence CCGG, but is generally unaffected by methylation at the internal cytosine. At specific subsets of this sequence such as the hexanucleotide CCGGCC, however, methylation of the internal cytosine strongly inhibits Msp I digestion, leading to artifacts in the interpretation of DNA methylation analyses. Our results show, for instance, that the CCGG site at the 5' end of the human gamma globin gene, which was thought to be methylated at both the internal and external cytosines, is actually methylated only at the internal CpG residue.
Keywords
BiologyMethylationCpG siteCytosineDNA methylationMolecular biology5-MethylcytosineDNAGeneticsMoietyGeneRestriction enzymeBiochemistryStereochemistryGene expressionChemistry
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Publication Info
- Year
- 1983
- Type
- article
- Volume
- 11
- Issue
- 11
- Pages
- 3571-3580
- Citations
- 62
- Access
- Closed
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Cite This
Elana Keshet,
Howard Cedar
(1983).
Effect of CpG methylation on<i>Msp</i>I.
Nucleic Acids Research
, 11
(11)
, 3571-3580.
https://doi.org/10.1093/nar/11.11.3571
Identifiers
- DOI
- 10.1093/nar/11.11.3571