The sequence GGCmCGG is resistant toMspI cleavage

Abstract

MspI essentially fails to cut the sequence GGCmCGG at enzyme concentrations which give total digestion of CCGG, CmCGG and GGCCGG sites. This result explains why certain sites in mammalian DNA are resistant to both MspI and HpaII and shows that this results from an idiosynchracy of MspI rather than a novel form of DNA methylation at this site in mammalian cells.

Keywords

HpaIIBiologyDNACleavage (geology)Restriction enzymeMolecular biologyMethylationSequence (biology)GeneticsDNA methylationGeneGene expression

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Publication Info

Year: 1983
Type: article
Volume: 11
Issue: 11
Pages: 3559-3569
Citations: 86
Access: Closed

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APA Style

                            
                                
                                    Meinrad Busslinger, 
                                
                                    Ernie de Boer, 
                                
                                    Sewall Wright
                                
                                et al.
                            
                            (1983). 
                            The sequence GGC<sup>m</sup>CGG is resistant to<i>Msp</i>I cleavage. 
                            Nucleic Acids Research
                            , 11
                            (11)
                            , 3559-3569.
                            https://doi.org/10.1093/nar/11.11.3559
                        

Identifiers

DOI: 10.1093/nar/11.11.3559