Superoxide Anion Radical (O·2), Superoxide Dismutases, and Related Matters

Abstract

A field of inquiry may be said to have come of age when conclusions initially viewed as remarkable or even unbelievable are accepted as commonplace. Study of the biology of the superoxide anion radical and of related free radicals, and the defenses thereto, has now reached this happy state of maturity. Superoxide and even hydroxyl radicals are now known to be produced in living systems, and elaborate systems of defense and repair, which minimize the ravages of these reactive species, have been described. New members of the superoxide dismutase, catalase, and peroxidase families of defensive enzymes are being found, as are new targets that are modified by O·̄2. In addition, the involvement of O·̄2 in both physiological and pathological processes is being established. A weighty tome would be needed to encompass a comprehensive coverage of this field of study. This review will describe only aspects of the biology of oxygen radicals that currently engage the interest of the writer. Hopefully they will also be of interest to the reader. Other recent reviews may serve to fill the gaps in this one (1Miller A.F. Sorkin D.L. Comments Mol. Cell. Biophys. 1997; 9: 1-48Google Scholar, 2Gardner, P. R. (1997) BioSci. Rep., in press.Google Scholar, 3McCord J.M. Proc. Soc. Exp. Biol. Med. 1995; 209: 112-117Crossref PubMed Scopus (136) Google Scholar, 4Valentine J.S. Ellerby L.M. Graden J.A. Nichida C.R. Gralla E.B. Kessissoglou D.P. Bioinorganic Chemistry. Kluwer Academic Publishers Group, Dordrecht, Netherlands1995: 77-91Crossref Google Scholar, 5Rosen G.M. Pou S. Ramos C.L. Cohen M.S. Britigan B.E. FASEB J. 1995; 9: 200-209Crossref PubMed Scopus (412) Google Scholar, 6Fridovich I. Annu. Rev. Biochem. 1995; 64: 97-112Crossref PubMed Scopus (2755) Google Scholar). Because of the spin restriction, the univalent reduction of O2 to O·̄2 is a facile process, and O·̄2production by spontaneous as well as enzyme-catalyzed reactions has been demonstrated. The instability of O·̄2 in aqueous solutions is a hindrance to its detection and measurement. This has been circumvented by exploiting its reaction with various "detector" molecules such as ferricytochrome c or spin-trapping agents. These agents are not specific for O·̄2. Thus, reductants other than O·̄2 can reduce ferricytochrome c, and oxidants other than O·̄2 can convert the spin traps to their epr-detectable hydroperoxy derivatives (7Moan J. Wold E. Nature. 1979; 279: 450-451Crossref PubMed Scopus (245) Google Scholar, 8Finkelstein E. Rosen G.M. Rauckman E.J. Arch. Biochem. Biophys. 1980; 200: 1-16Crossref PubMed Scopus (770) Google Scholar). Inhibition by SOD 1The abbreviations used are: SOD, superoxide dismutase; FALS, familial amyotrophic lateral sclerosis; EC-SOD, extracellular SOD. is used to lend specificity to these methods. Detection and measurement of fluxes of O·̄2 within cells is a goal as difficult as it is desirable. Unfortunately enthusiasm for achieving this goal has led many investigators to use flawed methods. An example is the luminescence that can be elicited from lucigenin. Early studies of the lucigenin luminescence elicited by the xanthine oxidase reaction led to the realization that the lucigenin dication must be univalently reduced to the corresponding monocation before reacting with O·̄2 in the process that leads to luminescence (9Greenlee L. Fridovich I. Handler P. Biochemistry. 1962; 1: 779-783Crossref PubMed Scopus (61) Google Scholar,10Allen R.C. Methods Enzymol. 1986; 13: 449-493Crossref Scopus (443) Google Scholar). The chemistry involved was discussed in a more recent review (11Faulkner K. Fridovich I. Free Radical Biol. & Med. 1993; 15: 447-451Crossref PubMed Scopus (329) Google Scholar). Nevertheless the use of lucigenin as a "specific" detector of O·̄2 continues. Quite recently it was shown that the lucigenin monocation radical can autoxidize and thus produce O·̄2, even in cases where no O·̄2 was being produced in the absence of lucigenin (12Liochev S.I. Fridovich I. Arch. Biochem. Biophys. 1997; 337: 115-120Crossref PubMed Scopus (203) Google Scholar). In studies with Escherichia coli, lucigenin was shown to function, much as does paraquat, to increase intracellular O·̄2 production (13Liochev S.I. Fridovich I. Proc. Natl. Acad. Sci. U. S. A. 1997; 94: 2891-2896Crossref PubMed Scopus (61) Google Scholar). Hopefully the widespread but inappropriate use of lucigenin luminescence as a measure of O·̄2 will stop. Another luminescent method that is misused to measure O·̄2 is based on luminol. In this case the compound must be univalently oxidized to a luminol radical, which reacts with O·̄2 before the light-emitting pathway is entered upon. The problem in this case is that the luminol radical can spontaneously reduce O2 to O·̄2. Luminol luminescence thus can be caused by a variety of oxidants and in all cases SOD inhibits (14Hodgson E.K. Fridovich I. Photochem. Photobiol. 1973; 18: 451-455Crossref PubMed Scopus (107) Google Scholar, 15Miller E.K. Fridovich I. J. Free Radicals Biol. & Med. 1986; 2: 107-110Crossref PubMed Scopus (25) Google Scholar). Here again the detector is acting as a source of O·̄2. One additional artifactual detector of O·̄2 needs to be mentioned because of its widespread misuse, and that is nitroblue tetrazolium. Many enzymes can cause the reduction of tetrazolium salts to the corresponding formazans. Reduction of nitroblue tetrazolium to the monoformazan requires two electrons and to the diformazan four electrons. When proceeding by a univalent pathway, which is usual, one encounters tetrazoinyl radical intermediates (16Bielski B.H.J. Shive G.G. Bajuk S. J. Phys. Chem. 1980; 84: 830-833Crossref Scopus (364) Google Scholar), which reduce O2 to O·̄2 in a reversible process. SOD by removing O·̄2 displaces this oxidation to the right and thus prevents production of the formazan. For this reason many aerobic tetrazolium reductions are inhibitable by SOD even though O·̄2 was not being produced in the system in the absence of the tetrazolium (17Liochev S.I. Fridovich I. Arch. Biochem. Biophys. 1995; 318: 408-410Crossref PubMed Scopus (58) Google Scholar). Is there any method which can reliably be used as a measure of intracellular O·̄2? There is and it is based on the rapid inactivation of [4Fe-4S]-containing dehydratases, such as aconitase. O·̄2 oxidizes the clusters of these dehydratases resulting in loss of Fe(II), and that is reversible by reduction and reincorporating of Fe(II). The balance between these opposing processes can be used as a measure of O·̄2 and has been so used in E. coli(18Gardner P.R. Fridovich I. J. Biol. Chem. 1992; 267: 8757-8763Abstract Full Text PDF PubMed Google Scholar) and in mammalian cells (19Gardner P.R. Raineri I. Epstein L.B. White C.W. J. Biol. Chem. 1995; 270: 13399-13405Abstract Full Text Full Text PDF PubMed Scopus (436) Google Scholar). The inactivation of aconitase by O·̄2 provides an explanation for previously inexplicable observations. Thus high pO2 (291 mm) increased both glucose utilization and lactate production, by 4–6-fold, in WI38 cells (20Balin A.K. Goodman D.B.P. Rasmussen H. Cristofalo V.J. J. Cell. Physiol. 1976; 89: 235-250Crossref PubMed Scopus (123) Google Scholar). Raising pO2 would increase O·̄2 production, and inactivation of aconitase by O·̄2 would force the cells to rely on fermentation of glucose for energy. In another exampleAspergillus niger was reported (21Kubicek C.P. Röhr M. Appl. Environ. Microbiol. 1985; 50: 1336-1338Crossref PubMed Google Scholar) to accumulate less citrate in the medium when supplied with 0.1 mg/liter Mn(II). In this instance enrichment of the medium with Mn(II) would increase the level of Mn-SOD in the mitochondria, and that in turn would decrease O·̄2 and thus raise aconitase. The final effect would be increased metabolism of citrate via the Krebs cycle and less citrate excretion. Near UV irradiation of E. coli inhibited growth on succinate more than growth on glucose (22Kashket E.R. Brodie A.F. J. Bacteriol. 1962; 83: 1094-1100Crossref PubMed Google Scholar) and inhibited respiration (23Swenson P.A. Schenley R.L. J. Bacteriol. 1974; 117: 551-559Crossref PubMed Google Scholar). Both of these effects can be explained by the inactivation of aconitase by photosensitized production of O·̄2. Aconitase can be inactivated by oxidants other than O·̄2. Of these peroxynitrite is particularly relevant to biology, but in NO-producing cells the level of peroxynitrite is itself dependent upon O·̄2 production. Inactivation by H2O2 is relatively unimportant. Although O·̄2 can initiate and propagate free radical oxidations of leukoflavins, tetrahydropterins, catecholamines, and related compounds and can inactivate [4Fe-4S]-containing dehydratases, it does not significantly attack polyunsaturated lipids or DNA. Yet defects in SODs, which would have the effect of raising intracellular [O·̄2], do lead to cell envelope damage (24Imlay J.A. Fridovich I. J. Bacteriol. 1992; 174: 953-961Crossref PubMed Google Scholar) and to enhanced mutagenesis (25Farr S.B. D'Ari R. Touati D. Proc. Natl. Acad. Sci. U. S. A. 1986; 83: 8268-8272Crossref PubMed Scopus (242) Google Scholar). Mechanisms by which O·̄2 can give rise to more potent oxidants could explain these seeming anomalies, and there are several such mechanisms. The simplest of these is protonation to hydroperoxyl radical, whose pK a is 4.8 and which is a much stronger oxidant than is O·̄2. Association of O·̄2 with other cationic centers such as vanadate (26Liochev S. Fridovich I. Arch. Biochem. Biophys. 1986; 250: 139-145Crossref PubMed Scopus (45) Google Scholar) or Mn(II) (27Curnutte J.T. Karnovsky M.L. Babior B.M. J. Clin. Invest. 1976; 57: 1059-1067Crossref PubMed Scopus (52) Google Scholar) also have this effect, but these mechanisms are unlikely to apply generally within cells. There is a mechanism pertinent to living cells, and we may call it thein vivo Haber-Weiss reaction. It is a process in which O·̄2 increase

Keywords

Affiliated Institutions

• Duke Medical Center US
• Duke University Hospital US

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