Keywords
BiologyFusion proteinEscherichia coliAffinity chromatographyGlutathione S-transferaseBiochemistryGlutathioneMolecular biologyExpression vectorRecombinant DNAAgarosePlasmidProteasesEnzymeGene
Affiliated Institutions
Related Publications
beta-Glucuronidase from Escherichia coli as a gene-fusion marker.
We have developed a gene-fusion system based on the Escherichia coli beta-glucuronidase gene (uidA). The uidA gene has been cloned from E. coli K-12 and its entire nucleotide se...
The Coronavirus Spike Protein Is a Class I Virus Fusion Protein: Structural and Functional Characterization of the Fusion Core Complex
ABSTRACT Coronavirus entry is mediated by the viral spike (S) glycoprotein. The 180-kDa oligomeric S protein of the murine coronavirus mouse hepatitis virus strain A59 is posttr...
USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products
We present a method that allows simultaneous fusion and cloning of multiple PCR products in a rapid and efficient manner. The procedure is based on the use of PCR primers that c...
Publication Info
- Year
- 1988
- Type
- article
- Volume
- 67
- Issue
- 1
- Pages
- 31-40
- Citations
- 5957
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
5957
OpenAlex
Cite This
Donald B. Smith,
Kevin S. Johnson
(1988).
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
Gene
, 67
(1)
, 31-40.
https://doi.org/10.1016/0378-1119(88)90005-4
Identifiers
- DOI
- 10.1016/0378-1119(88)90005-4