Berend‐Jan Bosch

The Coronavirus Spike Protein Is a Class I Virus Fusion Protein: Structural and Functional Characterization of the Fusion Core Complex

ABSTRACT Coronavirus entry is mediated by the viral spike (S) glycoprotein. The 180-kDa oligomeric S protein of the murine coronavirus mouse hepatitis virus strain A59 is posttr...

Tectonic conformational changes of a coronavirus spike glycoprotein promote membrane fusion

Significance The pandemic potential of coronaviruses was recently demonstrated twice by global outbreaks of deadly pneumonia. The spike (S) glycoprotein initiates infection thro...

Glycan shield and epitope masking of a coronavirus spike protein observed by cryo-electron microscopy

Cathepsin L Functionally Cleaves the Severe Acute Respiratory Syndrome Coronavirus Class I Fusion Protein Upstream of Rather than Adjacent to the Fusion Peptide

ABSTRACT Unlike other class I viral fusion proteins, spike proteins on severe acute respiratory sydrome coronavirus virions are uncleaved. As we and others have demonstrated, in...

Coronavirus Cell Entry Occurs through the Endo-/Lysosomal Pathway in a Proteolysis-Dependent Manner

Enveloped viruses need to fuse with a host cell membrane in order to deliver their genome into the host cell. While some viruses fuse with the plasma membrane, many viruses are ...

Human coronaviruses OC43 and HKU1 bind to 9- <i>O</i> -acetylated sialic acids via a conserved receptor-binding site in spike protein domain A

Human betacoronaviruses OC43 and HKU1 are endemic respiratory pathogens and, while related, originated from independent zoonotic introductions. OC43 is in fact a host-range vari...