Null mutation of copper/zinc superoxide dismutase in Drosophila confers hypersensitivity to paraquat and reduced longevity.

J. P. Phillips; Shelagh D. Campbell; Dominique Michaud; Michel Charbonneau; Arthur J. Hilliker

doi:10.1073/pnas.86.8.2761

Abstract

The role of copper/zinc-containing superoxide dismutase (cSOD; superoxide:superoxide oxidoreductase, EC 1.15.1.1) in metabolic defense against O2 toxicity in Drosophila is examined through the properties of a mutant strain carrying a cSOD-null mutation, cSODn108. Homozygotes are viable as larvae, which indicates that cSOD is not essential for cell viability per se. cSODn108 confers recessive sensitivity to the superoxide anion (O2-)-generator paraquat and to the transition metal compound CuSO4, which indicates that the cSOD-null condition in fact leads to impaired O2- metabolism. The primary biological consequences of the reduced O2- dismutation capacity of cSODn108 Drosophila are realized in the adult as infertility and reduction in life-span. We conclude that the infertility and reduced life-span of cSODn108 adults arise as a consequence of the reduced capacity of embryos, larvae, and pupae to adequately protect developing preimaginal cells from O2- -initiated cytotoxic damage.

Keywords

ParaquatSuperoxideSuperoxide dismutaseBiologyLongevityReactive oxygen speciesMutationBiochemistryGeneticsEnzymeGene

Affiliated Institutions

University of Guelph CA

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Publication Info

Year: 1989
Type: article
Volume: 86
Issue: 8
Pages: 2761-2765
Citations: 389
Access: Closed

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APA Style

                            
                                
                                    J. P. Phillips, 
                                
                                    Shelagh D. Campbell, 
                                
                                    Dominique Michaud
                                
                                et al.
                            
                            (1989). 
                            Null mutation of copper/zinc superoxide dismutase in Drosophila confers hypersensitivity to paraquat and reduced longevity.. 
                            Proceedings of the National Academy of Sciences
                            , 86
                            (8)
                            , 2761-2765.
                            https://doi.org/10.1073/pnas.86.8.2761
                        

Identifiers

DOI: 10.1073/pnas.86.8.2761