Abstract

ABSTRACT Use of the green fluorescent protein (Gfp) from the jellyfish Aequorea victoria is a powerful method for nondestructive in situ monitoring, since expression of green fluorescence does not require any substrate addition. To expand the use of Gfp as a reporter protein, new variants have been constructed by the addition of short peptide sequences to the C-terminal end of intact Gfp. This rendered the Gfp susceptible to the action of indigenous housekeeping proteases, resulting in protein variants with half-lives ranging from 40 min to a few hours when synthesized in Escherichia coli and Pseudomonas putida . The new Gfp variants should be useful for in situ studies of temporal gene expression.

Keywords

Green fluorescent proteinAequorea victoriaBiologyPseudomonas putidaEscherichia coliGene expressionGeneMolecular biologyBiochemistry

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Publication Info

Year
1998
Type
article
Volume
64
Issue
6
Pages
2240-2246
Citations
1037
Access
Closed

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Jens Bo Andersen, Claus Sternberg, Lars Poulsen et al. (1998). New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria. Applied and Environmental Microbiology , 64 (6) , 2240-2246. https://doi.org/10.1128/aem.64.6.2240-2246.1998

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DOI
10.1128/aem.64.6.2240-2246.1998