Abstract

A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic ( Escherichia coli ) or eukaryotic ( Caenorhabditis elegans ) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms.

Keywords

Green fluorescent proteinAequorea victoriaCaenorhabditis elegansEscherichia coliFluorescenceBiologyGeneGene expressionFluorescent proteinMolecular biologyProtein expressionProtein subcellular localization predictionProtein tagCell biologyBiochemistryFusion protein

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Publication Info

Year
1994
Type
article
Volume
263
Issue
5148
Pages
802-805
Citations
6686
Access
Closed

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Cite This

Martin Chalfie, Yuan Tu, Ghia Euskirchen et al. (1994). Green Fluorescent Protein as a Marker for Gene Expression. Science , 263 (5148) , 802-805. https://doi.org/10.1126/science.8303295

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DOI
10.1126/science.8303295