Abstract
We describe a technique for transferring electrophoretically separated bands of RNA from an agarose gel to paper strips. The RNA is coupled covalently to diazobenzyloxymethyl groups on the paper. After transfer and appropriate treatment of the paper to destroy remaining diazo groups, specific RNA bands can be detected by hybridization with 32P-labeled DNA probes followed by autoradiography. This procedure allows detection of specific RNA bands with high sensitivity and low background.
Keywords
AgaroseRNADNAMolecular biologyHybridization probeCovalent bondNucleic acid thermodynamicsDiazoChemistryBiologyBiochemistryGene
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Publication Info
- Year
- 1977
- Type
- article
- Volume
- 74
- Issue
- 12
- Pages
- 5350-5354
- Citations
- 2240
- Access
- Closed
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Cite This
James C. Alwine,
Darrell J. Kemp,
George R. Stark
(1977).
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes..
Proceedings of the National Academy of Sciences
, 74
(12)
, 5350-5354.
https://doi.org/10.1073/pnas.74.12.5350
Identifiers
- DOI
- 10.1073/pnas.74.12.5350