Abstract
A method has been developed for the electrophoretic transfer of DNA, RNA, protein and ribonucleoprotein particles from a variety of gels onto diazobenzyloxymethyl (DBM) - paper. Conditions for the electrophoretic transfer of these macromolecules have been optimized to allow for nearly quantitative transfer and covalent coupling. DNA and RNA electrophoretically transferred to DBM-paper retain their ability to hybridize with specific probes. The high efficiency of transfer and the high capacity of DBM-paper for nucleic acids makes possible the sensitive detection of specific nucleotide sequences. Similar efficiency is achieved in electrophoretic transfer and covalent coupling of proteins to DBM-paper. Macromolecules can also be electrophoretically transferred and bound to DBM-paper incapable of covalent bond formation. Their elution from the paper in high salt provides a new and useful preparative method for isolation of DNA, RNA and protein.
Keywords
BiologyNucleic acidRNADNALibrary scienceClassicsGeneticsHistoryGeneComputer science
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Publication Info
- Year
- 1980
- Type
- article
- Volume
- 8
- Issue
- 2
- Pages
- 299-317
- Citations
- 146
- Access
- Closed
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Cite This
Edmund J. Stellwag,
Albert E. Dahĺberg
(1980).
Electrophoretic transfer of DNA, RNA and protein onto diazobenzyloxymethyl (DBM) - paper.
Nucleic Acids Research
, 8
(2)
, 299-317.
https://doi.org/10.1093/nar/8.2.299
Identifiers
- DOI
- 10.1093/nar/8.2.299