Abstract
Abstract Advances in sequencing technology allow genomes to be sequenced at vastly decreased costs. However, the assembled data frequently are highly fragmented with many gaps. We present a practical approach that uses Illumina sequences to improve draft genome assemblies by aligning sequences against contig ends and performing local assemblies to produce gap-spanning contigs. The continuity of a draft genome can thus be substantially improved, often without the need to generate new data.
Keywords
ContigGenomeSequence assemblyBiologyComputational biologyHybrid genome assemblyGeneticsGeneTranscriptome
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Publication Info
- Year
- 2010
- Type
- article
- Volume
- 11
- Issue
- 4
- Pages
- R41-R41
- Citations
- 289
- Access
- Closed
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Cite This
Isheng Jason Tsai,
Thomas D. Otto,
Matthew Berriman
(2010).
Improving draft assemblies by iterative mapping and assembly of short reads to eliminate gaps.
Genome biology
, 11
(4)
, R41-R41.
https://doi.org/10.1186/gb-2010-11-4-r41
Identifiers
- DOI
- 10.1186/gb-2010-11-4-r41