Abstract

Abstract We have identified and purified a factor that inhibits the production of IL-1 beta and TNF by stimulated human mononuclear cells. The activity is produced by the T cell lines Hut-78 and Mo constitutively under serum-free conditions. Crude conditioned media have titers of up to 100 U/ml (one unit defined as the reciprocal of the dilution producing 50% inhibition). The activity resides mainly in a single size peak of 30 to 35 kDa and an isoelectric point around 8. Other cytokines in this size range that have been reported to be inhibitory for IL-1 and TNF production include TGF-beta, IL-4, and IL-6; these factors were excluded by lack of detection, neutralizing antibody, and low activity compared with our factor. Another factor with these size and charge properties is IL-10, which inhibits T cell cytokine production. By polymerase chain reaction analysis, Mo and HuT-78 lines contain IL-10 transcripts whereas JURKAT is negative; this correlates with inhibitor bioactivity from the three lines. Use of mAb specifically showed the inhibitor to be IL-10.

Keywords

Molecular biologyJurkat cellsPeripheral blood mononuclear cellTumor necrosis factor alphaIsoelectric pointCytokineCell cultureAntibodyBiologyT cellLymphocyteChemistryImmunologyIn vitroBiochemistryImmune systemEnzyme

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Publication Info

Year
1992
Type
article
Volume
148
Issue
3
Pages
808-814
Citations
226
Access
Closed

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P Ralph, Ilona Nakoinz, Adam Sampson-Johannes et al. (1992). IL-10, T lymphocyte inhibitor of human blood cell production of IL-1 and tumor necrosis factor. The Journal of Immunology , 148 (3) , 808-814. https://doi.org/10.4049/jimmunol.148.3.808

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DOI
10.4049/jimmunol.148.3.808