Abstract
Culture of human peripheral blood mononuclear cells (PBMC) with IL-2 stimulates synthesis of cytokines and generation of lymphokine-activated killer (LAK) activity. Both IL-4 and IL-10 [cytokine synthesis inhibitory factor (CSIF)] inhibit IL-2-induced synthesis of IFN-gamma and tumor necrosis factor (TNF)-alpha by human PBMC. However, unlike IL-4, IL-10 inhibits neither IL-2-induced proliferation of PBMC and fresh natural killer (NK) cells, nor IL-2-induced LAK activity. Moreover, IL-4 inhibits IL-2-induced IFN-gamma synthesis by purified fresh NK cells, while in contrast the inhibitory effect of IL-10 is mediated by CD14+ cells (monocytes/macrophages). IL-10 inhibits TNF-alpha synthesis by monocytes or monocytes plus NK cells, but not by NK cells alone. These results suggest that IL-4 and IL-10 act on NK cells via distinct pathways, and that IL-2-induced cytokine synthesis and LAK activity are regulated via different mechanisms.
Keywords
Lymphokine-activated killer cellLymphokineCytokineInterleukin 12Peripheral blood mononuclear cellTumor necrosis factor alphaNatural killer T cellChemistryInterleukin 2Interleukin 21ImmunologyBiologyMolecular biologyCytotoxic T cellT cellImmune systemIn vitroBiochemistry
Related Publications
Plasma inflammatory cytokines and chemokines in severe acute respiratory syndrome
SUMMARY Severe acute respiratory syndrome (SARS) is a recently emerged infectious disease caused by a novel coronavirus, but its immunopathological mechanisms have not yet been ...
Blockade of Interleukin-6 Signaling Suppresses Not Only Th17 but Also Interphotoreceptor Retinoid Binding Protein–Specific Th1 by Promoting Regulatory T Cells in Experimental Autoimmune Uveoretinitis
PURPOSE. Both Th17 and Th1 cells contribute to experimental autoimmune uveoretinitis (EAU). Interleukin-6 (IL-6) blockade inhibits Th17 differentiation in EAU and potently suppr...
Interleukin 6 Is Required for the Development of Collagen-induced Arthritis
Interleukin-6 (IL-6) is overproduced in the joints of patients with rheumatoid arthritis (RA) and, based on its multiple stimulatory effects on cells of the immune system and on...
Macrophage plasticity, polarization, and function in health and disease
Macrophages are heterogeneous and their phenotype and functions are regulated by the surrounding micro‐environment. Macrophages commonly exist in two distinct subsets: 1) Classi...
Macrophage Activation-Like Syndrome: A Distinct Entity Leading to Early Death in Sepsis
Hemophagocytic lymphohistocytosis (HLH) is characterized by fulminant cytokine storm leading to multiple organ dysfunction and high mortality. HLH is classified into familial (f...
Publication Info
- Year
- 1992
- Type
- article
- Volume
- 4
- Issue
- 5
- Pages
- 563-569
- Citations
- 206
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
206
OpenAlex
Cite This
Di-Hwel Hsu,
Kevin W. Moore,
Hergen Splts
(1992).
Differential effects of IL-4 and IL-10 on IL-2-induced IFN-γ synthesis and lymphokine-activated killer activity.
International Immunology
, 4
(5)
, 563-569.
https://doi.org/10.1093/intimm/4.5.563
Identifiers
- DOI
- 10.1093/intimm/4.5.563