Abstract
A physical map of the genome of Clostridium perfringens, an important human pathogen, has been established by pulsed-field gel electrophoresis. Recognition sites for six rare-cutting endonucleases were situated on a single circular chromosome of approximately 3.6 million base pairs thus defining 50 arbitrary genetic intervals of between 10 and 250 kilobase pairs. This considerably facilitated the chromosomal localization of some 24 genes and loci for which probes were available and allowed the construction of the genome map of a clostridial species.
Keywords
Clostridium perfringensGenomeBiologyGeneticsChromosomeGenePathogenClostridiumComputational biologyGenome sizeBacteria
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Publication Info
- Year
- 1989
- Type
- article
- Volume
- 86
- Issue
- 17
- Pages
- 6676-6680
- Citations
- 163
- Access
- Closed
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Cite This
Bruno Canard,
S T Cole
(1989).
Genome organization of the anaerobic pathogen Clostridium perfringens..
Proceedings of the National Academy of Sciences
, 86
(17)
, 6676-6680.
https://doi.org/10.1073/pnas.86.17.6676
Identifiers
- DOI
- 10.1073/pnas.86.17.6676