Abstract
We constructed the physical map of Chlamydia trachomatis serovar L2 by using three restriction endonucleases, NotI (GC[GGCCGC), SgrAI (C(A/G)[CCGG(T/G)G), and Sse8387I (CCTGCA[GG), and we analyzed the fragments by pulsed-field gel electrophoresis. A total of 25 restriction endonuclease sites and 13 genes and/or operons were located on the map. The genome size was determined to be 1,045 kb. Neither highly transcribed chlamydia genes nor developmental cycle-specific genes were clustered on the genome.
Keywords
BiologyChlamydia trachomatisRestriction enzymeGenomeGel electrophoresisRestriction mapGeneticsGeneSerotypeMolecular biologyChlamydiaceaeOperonRestriction fragment length polymorphismRestriction fragmentPulsed-field gel electrophoresisGenome sizePolymerase chain reactionVirologyGenotypeNucleic acid sequenceEscherichia coli
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Publication Info
- Year
- 1992
- Type
- article
- Volume
- 174
- Issue
- 9
- Pages
- 2742-2747
- Citations
- 54
- Access
- Closed
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Cite This
Svend Birkelund,
Richard S. Stephens
(1992).
Construction of physical and genetic maps of Chlamydia trachomatis serovar L2 by pulsed-field gel electrophoresis.
Journal of Bacteriology
, 174
(9)
, 2742-2747.
https://doi.org/10.1128/jb.174.9.2742-2747.1992
Identifiers
- DOI
- 10.1128/jb.174.9.2742-2747.1992