Figure 4 from Phase Separation Mediates NUP98 Fusion Oncoprotein Leukemic Transformation

Bappaditya Chandra , Nicole L. Michmerhuizen , Hazheen K. Shirnekhi , Bappaditya Chandra , Nicole L. Michmerhuizen , Hazheen K. Shirnekhi , Swarnendu Tripathi , Brittany J. Pioso , David W. Baggett , Diana M. Mitrea , Ilaria Iacobucci , Michael R. White , Jingjing Chen , Cheon‐Gil Park , Huiyun Wu , Stanley Pounds , Anna Medyukhina , Khaled Khairy , Qingsong Gao , Chunxu Qu , Sherif Abdelhamed , Scott D. Gorman , Simranjot Bawa , Carolyn Maslanka , Swati Kinger , Priyanka Dogra , Mylene C. Ferrolino , Danika Di Giacomo , Cristina Mecucci , Jeffery M. Klco , Charles G. Mullighan , Richard W. Kriwacki
2025 0 citations

Abstract

<p>Mutation of F and FG residues in the FG-rich IDR of NHA9 alters LLPS behavior <i>in vitro</i>. <b>A–C,</b> Confocal fluorescence micrographs of Alexa 488–labeled NHA9–8FA (<b>A</b>), NHA9–21FGAA (<b>B</b>), and NHA9<sub>Midi</sub> (<b>C</b>) condensates <i>in vitro</i> with increasing protein concentration. Micrographs are presented as maximum intensity projections of 13 Z-stack images acquired over 6 μm with 0.5 μm resolution. <b>D,</b> Confocal micrograph of Alexa 488–labeled NHA9<sub>Midi</sub>–21FGAA at a concentration of 20 μmol/L. Saturation concentration (<i>C</i><sub>sat</sub>) is less than 10 nmol/L (<b>A</b>), between 160 nmol/L and 315 nmol/L (<b>B</b>), and between 40 nmol/L and 80 nmol/L (<b>C</b>).</p>

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2025
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Bappaditya Chandra, Nicole L. Michmerhuizen, Hazheen K. Shirnekhi et al. (2025). Figure 4 from Phase Separation Mediates NUP98 Fusion Oncoprotein Leukemic Transformation. . https://doi.org/10.1158/2159-8290.30834176

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10.1158/2159-8290.30834176