Abstract
Epstein-Barr virus (EBV) latently infects and immortalizes B lymphocytes and causes lymphoproliferative malignancies. We show here that the EBV nuclear antigen EBNA2 induces expression of the 2 chains of the interleukin-18 receptor (IL-18R) in Burkitt lymphoma (BL) cell lines and in nontransformed B cells. Activation of IL-18R expression by EBNA2 is independent of its interaction with the transcriptional repressor RBPJ kappa. It occurs in the absence of any other viral protein but requires de novo synthesis of cellular proteins. IL-18R induction is a highly specific function of EBNA2, because neither other EBV latent proteins nor the cellular proteins c-myc or Notch can exert this effect. Using cDNA microarray expression profiling, we find that the IL-18 receptor expressed in EBV-infected BL cells has signaling capacity, because IL-18 significantly modified gene expression. We report that EBNA2 expression is associated with IL-18R expression in vivo in EBV-positive B-lymphomas from AIDS patients.
Keywords
BiologyMolecular biologyEpstein–Barr virusVirusAntigenB cellGene expressionVirologyGeneImmunologyAntibody
Affiliated Institutions
- Centre National de la Recherche Scientifique FR
- Hôpital Avicenne FR
- Hôtel-Dieu de Paris FR
- Assistance Publique – Hôpitaux de Paris FR
- Inserm FR
- Centre de Recherche des Cordeliers FR
- Hôpital Européen FR
- Sorbonne Université FR
- Urologische Klinik München DE
- Institut Gustave Roussy FR
- Hôpital Européen Georges-Pompidou FR
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Publication Info
- Year
- 2004
- Type
- article
- Volume
- 105
- Issue
- 4
- Pages
- 1632-1639
- Citations
- 27
- Access
- Closed
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Cite This
Franck Pagès
(2004).
Epstein-Barr virus nuclear antigen 2 induces interleukin-18 receptor expression in B cells.
Blood
, 105
(4)
, 1632-1639.
https://doi.org/10.1182/blood-2004-08-3196
Identifiers
- DOI
- 10.1182/blood-2004-08-3196