Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI.

Abstract

We have demonstrated the existence of human cytokine synthesis inhibitory factor (CSIF) [interleukin 10 (IL-10)]. cDNA clones encoding human IL-10 (hIL-10) were isolated from a tetanus toxin-specific human T-cell clone. Like mouse IL-10, hIL-10 exhibits strong DNA and amino acid sequence homology to an open reading frame in the Epstein-Barr virus, BCRFI. hIL-10 and the BCRFI product inhibit cytokine synthesis by activated human peripheral blood mononuclear cells and by a mouse Th1 clone. Both hIL-10 and mouse IL-10 sustain the viability of a mouse mast cell line in culture, but BCRFI lacks comparable activity in this assay, suggesting that BCRFI may have conserved only a subset of hIL-10 activities.

Keywords

BiologyOpen reading frameComplementary DNAMolecular biologyVirologyHomology (biology)CytokinePeptide sequenceVirusGeneGenetics

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Publication Info

Year: 1991
Type: article
Volume: 88
Issue: 4
Pages: 1172-1176
Citations: 751
Access: Closed

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APA Style

                            
                                
                                    Paulo Vieira, 
                                
                                    René de Waal Malefyt, 
                                
                                    M N Dang
                                
                                et al.
                            
                            (1991). 
                            Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI.. 
                            Proceedings of the National Academy of Sciences
                            , 88
                            (4)
                            , 1172-1176.
                            https://doi.org/10.1073/pnas.88.4.1172
                        

Identifiers

DOI: 10.1073/pnas.88.4.1172