Cloning of the cDNA and gene for a human D2 dopamine receptor.

Abstract

A clone encoding a human D2 dopamine receptor was isolated from a pituitary cDNA library and sequenced. The deduced protein sequence is 96% identical with that of the cloned rat receptor with one major difference: the human receptor contains an additional 29 amino acids in its putative third cytoplasmic loop. Southern blotting demonstrated the presence of only one human D2 receptor gene. Two overlapping phage containing the gene were isolated and characterized. DNA sequence analysis of these clones showed that the coding sequence is interrupted by six introns and that the additional amino acids present in the human pituitary receptor are encoded by a single exon of 87 base pairs. The involvement of this sequence in alternative splicing and its biological significance are discussed.

Keywords

BiologyComplementary DNAExonGeneMolecular biologyCoding regionGeneticsIntronPeptide sequencecDNA library5-HT5A receptorNucleic acid sequenceAlternative splicing

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Publication Info

Year: 1989
Type: article
Volume: 86
Issue: 24
Pages: 9762-9766
Citations: 487
Access: Closed

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APA Style

                            
                                
                                    David K. Grandy, 
                                
                                    Mark A. Marchionni, 
                                
                                    H Makam
                                
                                et al.
                            
                            (1989). 
                            Cloning of the cDNA and gene for a human D2 dopamine receptor.. 
                            Proceedings of the National Academy of Sciences
                            , 86
                            (24)
                            , 9762-9766.
                            https://doi.org/10.1073/pnas.86.24.9762
                        

Identifiers

DOI: 10.1073/pnas.86.24.9762