Abstract
CD28 (Tp44) is a human T-cell-specific homodimer surface protein that may participate in T-cell activation. We have isolated a cDNA clone encoding CD28 by a simple and highly efficient cloning strategy based on transient expression in COS cells. Central to this strategy is the use of an efficient method to prepare large plasmid cDNA libraries. The libraries are introduced into COS cells, where transient expression of surface antigen allows the isolation of cDNAs by way of monoclonal antibody binding. The CD28 cDNA encodes a highly glycosylated membrane protein with homology to the immunoglobulin superfamily and directs the production of a homodimer in transfected COS cells.
Keywords
Expression cloningComplementary DNAMolecular biologyCD28BiologyTransfectioncDNA libraryPlasmidCloning (programming)Molecular cloningMonoclonal antibodyExpression vectorAntigenGeneAntibodyRecombinant DNAGenetics
Affiliated Institutions
Related Publications
Point mutations impairing cell surface expression of the common beta subunit (CD18) in a patient with leukocyte adhesion molecule (Leu-CAM) deficiency.
The leukocyte adhesion molecules CD11a/CD18, CD11b/CD18, and CD11c/CD18 (Leu-CAM) are members of the integrin receptor family and mediate crucial adhesion-dependent functions in...
T cell receptor-dependent, antigen-specific stimulation of a murine T cell clone induces a transient, VLA protein-mediated binding to extracellular matrix
Abstract Upon Ag stimulation, an arsonate-specific murine T cell clone exhibited a rapid but transient increase in cell adhesion to collagen, fibronectin, and laminin. This incr...
Biological behavior of human breast carcinoma-associated antigens expressed during cellular proliferation.
The cell surface-binding properties of two murine monoclonal antibodies reactive with human mammary tumor cells are described. Fluorescence-activated cell sorter analyses demons...
Mouse Embryonic Stem Cells and Reporter Constructs to Detect Developmentally Regulated Genes
A strategy was devised for identifying regions of the mouse genome that are transcriptionally active in a temporally and spatially restricted manner during development. The appr...
Somatic hypermutation in normal and transformed human B cells
Summary: In the human, most IgM + IgD + as well as CD5* peripheral blood B cells express unmutated V genes and thus can be assigned to a pre‐germinal centre (GC) stage of develo...
Publication Info
- Year
- 1987
- Type
- article
- Volume
- 84
- Issue
- 23
- Pages
- 8573-8577
- Citations
- 790
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
790
OpenAlex
Cite This
A Aruffo,
Brian Seed
(1987).
Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system..
Proceedings of the National Academy of Sciences
, 84
(23)
, 8573-8577.
https://doi.org/10.1073/pnas.84.23.8573
Identifiers
- DOI
- 10.1073/pnas.84.23.8573