A novel gene encoding a B-box protein within the BRCA1 region at 17q21.1

Ian Campbell; Hans M. Nicolal; William D. Foulkes; abriel Senger; Gordon W. Stamps; Gordon Allans; Clnda Boyer; Karen A. Jones; Robert C. Bast; Ellen Solomon; John Trowsdale; Donald M. Black

doi:10.1093/hmg/3.4.589

Abstract

A novel cDNA clone was isolated using a polyclonal serum directed against partially purified ovarian carcinoma antigen CA125. The deduced peptide sequence lacked membrane protein characteristics expected for CA125 but encompassed a B-box/coiled coil motif present in many genes with transformation potential. The gene was mapped by fluorescence in situ hybridization within the minimum region known to contain the familial breast/ovarian carcinoma gene, BRCA1. YAC and cosmid clones were isolated and used to refine the location of this gene adjacent and proximal to the RNU2 locus. The exon structure of the gene was determined. Extensive SSCP and sequence analysis of over 100 tumour and normal DNAs from familial and sporadic breast cancers and sporadic ovarian cancers failed to detect mutations in the coding region of this gene.

Keywords

BiologyGeneMolecular biologyExonCosmidCoding regionComplementary DNAGeneticsSingle-strand conformation polymorphismLocus (genetics)Gene mappingChromosome

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Publication Info

Year: 1994
Type: article
Volume: 3
Issue: 4
Pages: 589-594
Citations: 99
Access: Closed

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APA Style

                            
                                
                                    Ian Campbell, 
                                
                                    Hans M. Nicolal, 
                                
                                    William D. Foulkes
                                
                                et al.
                            
                            (1994). 
                            A novel gene encoding a B-box protein within the BRCA1 region at 17q21.1. 
                            Human Molecular Genetics
                            , 3
                            (4)
                            , 589-594.
                            https://doi.org/10.1093/hmg/3.4.589
                        

Identifiers

DOI: 10.1093/hmg/3.4.589