R. Bruce Wallace

Prenatal Diagnosis of β-Thalassemia

We investigated a method employing synthetic oligonucleotides for the prenatal diagnosis of beta-thalassemia due to a single nucleotide mutation. The beta 0 thalassemia we teste...

α1-Antitrypsin deficiency detection by direct analysis of the mutation in the gene

A deficiency in the plasma protease inhibitor alpha 1-antitrypsin can cause chronic obstructive emphysema or infantile liver cirrhosis. This deficiency results from a single ami...

Identification of the Cloned Gene for the Murine Transplantation Antigen H-2K^bby Hybridization with Synthetic Oligonucleotides

The H-2K b gene is a member of the large major histocompatibility complex class I gene family. Since many members of this family cross-hybridize with class I cDNA probes, the cl...

Directed Deletion of a Yeast Transfer RNA Intervening Sequence

Many eukaryotic genes contain intervening sequences, segments of DNA that interrupt the continuity of the gene. They are removed from RNA transcripts of the gene by a process kn...

A set of synthetic oligodeoxyribonucleotide primers for DNA sequencing in the plasmid vector pBR322