Identification of the Cloned Gene for the Murine Transplantation Antigen H-2Kbby Hybridization with Synthetic Oligonucleotides

Dan H. Schulze; Larry R. Pease; Yuichi Obata; Stanley G. Nathenson; Antonio A. Reyes; Satoshi Ikuta; R. Bruce Wallace

doi:10.1128/mcb.3.4.750

Abstract

The H-2K b gene is a member of the large major histocompatibility complex class I gene family. Since many members of this family cross-hybridize with class I cDNA probes, the cloned H-2K b gene was identified by hybridization with specific oligonucleotide probes. This clone was definitively shown to encode the H-2K b polypeptide by partial DNA sequencing and by serological and tryptic peptide analyses of the expressed product.

Keywords

BiologyComplementary DNAOligonucleotideMolecular biologyGeneMajor histocompatibility complexclone (Java method)Nucleic acid thermodynamicsDNAGeneticsRNA

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Publication Info

Year: 1983
Type: article
Volume: 3
Issue: 4
Pages: 750-755
Citations: 38
Access: Closed

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APA Style

                            
                                
                                    Dan H. Schulze, 
                                
                                    Larry R. Pease, 
                                
                                    Yuichi Obata
                                
                                et al.
                            
                            (1983). 
                            Identification of the Cloned Gene for the Murine Transplantation Antigen H-2K<sup>b</sup>by Hybridization with Synthetic Oligonucleotides. 
                            Molecular and Cellular Biology
                            , 3
                            (4)
                            , 750-755.
                            https://doi.org/10.1128/mcb.3.4.750
                        

Identifiers

DOI: 10.1128/mcb.3.4.750