Karel Svoboda

Genetic Dissection of Neural Circuits

Rapid and Reversible Chemical Inactivation of Synaptic Transmission in Genetically Targeted Neurons

A Cre-Dependent GCaMP3 Reporter Mouse for Neuronal Imaging<i>In Vivo</i>

Fluorescent calcium indicator proteins, such as GCaMP3, allow imaging of activity in genetically defined neuronal populations. GCaMP3 can be expressed using various gene deliver...

Activity in motor–sensory projections reveals distributed coding in somatosensation

Monitoring Neural Activity and [Ca²⁺] with Genetically Encoded Ca²⁺Indicators

Genetically encoded Ca 2+ indicators (GECIs) based on fluorescent proteins (XFPs) and Ca 2+ -binding proteins [like calmodulin (CaM)] have great potential for the study of subce...

Stereotyped Odor-Evoked Activity in the Mushroom Body of <i>Drosophila</i> Revealed by Green Fluorescent Protein-Based Ca ²⁺ Imaging

To study the representation of olfactory information in higher brain centers, we expressed a green fluorescent protein-based Ca 2+ sensor, G-CaMP, in the Drosophila mushroom bod...

Characterization and Subcellular Targeting of GCaMP-Type Genetically-Encoded Calcium Indicators

Genetically-encoded calcium indicators (GECIs) hold the promise of monitoring [Ca(2+)] in selected populations of neurons and in specific cellular compartments. Relating GECI fl...

Shared and distinct transcriptomic cell types across neocortical areas

The neocortex contains a multitude of cell types that are segregated into layers and functionally distinct areas. To investigate the diversity of cell types across the mouse neo...

Experience-dependent and cell-type-specific spine growth in the neocortex

Ultrasensitive fluorescent proteins for imaging neuronal activity

Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators

Optimization of a GCaMP Calcium Indicator for Neural Activity Imaging

Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Recent efforts in protein engineering have significantly increased the performance of...

In vivo dendritic calcium dynamics in neocortical pyramidal neurons

The Life Cycle of Ca2+ Ions in Dendritic Spines

Multiple dynamic representations in the motor cortex during sensorimotor learning