Abstract

Nucleosome core particles containing a DNA palindrome and purified chicken erythrocyte histone octamer have been reconstituted and crystallized. The dyad symmetry of the palindrome extends the dyad symmetry of the histone octamer to result in a twofold symmetric particle. This ensures that the structure determined by X-ray diffraction will yield a true representation of the DNA strand rather than the twofold averaged structure which would result from using a non-palindromic DNA sequence. The crystals provide isotropic diffraction to 3.2 A with observed reflections extending to d spacings of about 2.8 A using a rotating-anode Cu Kalpha X-ray source. Although the DNA palindrome is a factor contributing to the quality of the diffraction data, another significant factor is an improved preparative technique which enriches for correctly phased nucleosome core particles.

Keywords

Histone octamerNucleosomeCrystallographyDiffractionDNAHistonePhysicsPalindromic sequenceSuperstructureChemistryMolecular physicsBiophysicsPalindromeOpticsBiologyThermodynamicsBiochemistry

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Year
1996
Type
article
Volume
52
Issue
2
Pages
283-288
Citations
32
Access
Closed

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Joel M. Harp, Ed Uberbacher, Arthur E. Roberson et al. (1996). X-ray Diffraction Analysis of Crystals Containing Twofold Symmetric Nucleosome Core Particles. Acta Crystallographica Section D Biological Crystallography , 52 (2) , 283-288. https://doi.org/10.1107/s0907444995009139

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DOI
10.1107/s0907444995009139