Abstract

The nucleosome is the universal protein/DNA element repeated throughout the chromatin of eukaryotic cells (Kornberg 1977; McGhee and Felsenfeld 1980). Presumably as a consequence of its fundamental role in the organization of DNA in a compact unit, it has also been found to take part in gene regulation (see, e.g., Pederson et al. 1986; van Holde 1988; Grunstein 1990; Wolffe 1991; Kornberg and Lorch 1992). A major objective of our laboratory has been to obtain structural information at high resolution for the nucleosome, its higher-order structure, and its interactions with other nuclear factors. Previously, the structure of the nucleosome core particle was solved by X-ray crystallography at 7 Å resolution using the method of multiple isomorphous replacement (Richmond et al. 1984). This X-ray structure confirmed the size and shape of the particle deduced earlier from several lines of information (Finch et al. 1979), showing that the "146"-bp DNA is arranged...

Keywords

NucleosomeChromatinDNAGeneticsBiologyChemistryBiophysicsPhysicsCrystallographyMolecular biology

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Year
1993
Type
article
Volume
58
Issue
0
Pages
265-272
Citations
46
Access
Closed

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Timothy J. Richmond, Thomas Rechsteiner, Karolin Luger (1993). Studies of Nucleosome Structure. Cold Spring Harbor Symposia on Quantitative Biology , 58 (0) , 265-272. https://doi.org/10.1101/sqb.1993.058.01.031

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DOI
10.1101/sqb.1993.058.01.031