Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content.

Abstract

A PCR method for uniform amplification of a mixture of DNA templates differing in GC content is described using the two enzyme approach (Klentaq1 and Pfu DNA polymerase) and a combination of DMSO and betaine. This method was applied to amplify the CGG repeat region from the fragile X region.

Keywords

BiologyGC-contentMultiple displacement amplificationDNAPolymerase chain reactionBetaineMolecular biologyPolymeraseGeneticsBiochemistryDNA extractionGeneGenome

Affiliated Institutions

Yale University US

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Publication Info

Year: 1996
Type: article
Volume: 6
Issue: 7
Pages: 633-638
Citations: 174
Access: Closed

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APA Style

                            
                                
                                    Namadev Baskaran, 
                                
                                    Rajendra P. Kandpal, 
                                
                                    A.K. Bhargava
                                
                                et al.
                            
                            (1996). 
                            Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content.. 
                            Genome Research
                            , 6
                            (7)
                            , 633-638.
                            https://doi.org/10.1101/gr.6.7.633
                        

Identifiers

DOI: 10.1101/gr.6.7.633