Hamilton O. Smith

Enzymatic assembly of DNA molecules up to several hundred kilobases

Complete Chemical Synthesis, Assembly, and Cloning of a <i>Mycoplasma genitalium</i> Genome

We have synthesized a 582,970–base pair Mycoplasma genitalium genome. This synthetic genome, named M. genitalium JCVI-1.0, contains all the genes of wild-type M. genitalium G37 ...

A Restriction enzyme from Hemophilus influenzae

Cell-free cloning using φ29 DNA polymerase

We describe conditions for rolling-circle amplification (RCA) of individual DNA molecules 5–7 kb in size by &gt;10 9 -fold, using φ29 DNA polymerase. The principal difficulty wi...

The Sequence of the Human Genome

A 2.91-billion base pair (bp) consensus sequence of the euchromatic portion of the human genome was generated by the whole-genome shotgun sequencing method. The 14.8-billion bp ...

Complete Genome Sequence of a Virulent Isolate of <i>Streptococcus pneumoniae</i>

The 2,160,837–base pair genome sequence of an isolate of Streptococcus pneumoniae , a Gram-positive pathogen that causes pneumonia, bacteremia, meningitis, and otitis media, con...

Restriction Endonucleases in the Analysis and Restructuring of DNA Molecules

The Hippo pathway was initially discovered in Drosophila melanogaster as a key regulator of tissue growth. It is an evolutionarily conserved signaling cascade regulating numerou...

The Sorcerer II Global Ocean Sampling Expedition: Northwest Atlantic through Eastern Tropical Pacific

The world's oceans contain a complex mixture of micro-organisms that are for the most part, uncharacterized both genetically and biochemically. We report here a metagenomic stud...