Abstract
Seven vitamins have to date proved essential for the survival and multiplication of a mouse fibroblast (strain L) and a human carcinoma cell (strain HeLa) in tissue culture: choline, folic acid, nicotinamide, pantothenic acid, pyridoxal, riboflavin, and thiamin. It was necessary to cultivate the cells for 5 to 15 days in a medium lacking the specific vitamin before the deficiency became apparent in the cessation of multiplication and the development of specific cytopathogenic effects. In their early stages these changes could be reversed by the addition of the missing vitamin, an in vitro, "cure" of a vitamin deficiency. The maximally effective concentrations were in the range 107 to 108 gm. per ml. The probability that additional vitamins not demonstrably essential under the conditions of the present experiments are nevertheless required for survival and growth is discussed in the text.
Keywords
Pantothenic acidVitaminThiamineRiboflavinIn vitroBiologyPyridoxineNicotinamideFibroblastBiochemistryTissue cultureAscorbic acidChemically defined mediumChemistryFood scienceEnzyme
Affiliated Institutions
Related Publications
Genetic mapping of a mutation that causes ribonucleases III deficiency in Escherichia coli
the mutation that causes ribonuclease III (RNase III) deficiency in strain AB301-105 of Kindler et al. (1973) has been mapped by use of F' merodiploids, Hfr matings, and P1 tran...
Synthesis of Antihemophilic Factor Antigen by Cultured Human Endothelial Cells
Antihemophilic factor (AHF, Factor VIII) antigen has been demonstrated in cultured human endothelial cells by immunofluorescence studies using monospecific rabbit antibody to hu...
Effect of Dietary Selenium Dioxide, Cystine, Ethoxyquin and Vitamin E on Lipid Autoxidation in Chick Tissues
Abstract The ability of dictary selenium dioxide, L‐cystine, ethoxyquin (1,2‐dihydro‐6‐ethoxy‐2,2,4‐trimethylquinoline) and dl‐α‐tocopheryl acetate to inhibit autoxidation in in...
[Scavenging effects of probimane on active oxygen free radicals by electron spin resonance].
Probimane, dl-1,2-bis (4-morpholine-methyl-3, 5-dioxopiperazin-1-yl) propane, is a new antitumor agent synthesized by Shanghai Institute of Materia Medica, Chinese Academy of Sc...
COLOMINIC ACID, A POLYMER OF N-ACETYLNEURAMINIC ACID
All acidic carbohydrate has been isolated from the culture medium of the enteric microorganism E. coli K235 L+O. This substance has been named colominic acid. N-acetylneuraminic...
Publication Info
- Year
- 1955
- Type
- article
- Volume
- 102
- Issue
- 5
- Pages
- 595-600
- Citations
- 322
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
322
OpenAlex
Cite This
Harry Eagle
(1955).
THE MINIMUM VITAMIN REQUIREMENTS OF THE L AND H<scp>E</scp>L<scp>A</scp> CELLS IN TISSUE CULTURE, THE PRODUCTION OF SPECIFIC VITAMIN DEFICIENCIES, AND THEIR CURE.
The Journal of Experimental Medicine
, 102
(5)
, 595-600.
https://doi.org/10.1084/jem.102.5.595
Identifiers
- DOI
- 10.1084/jem.102.5.595