The Complete Genome Sequence of Escherichia coli K-12

Abstract

The 4,639,221–base pair sequence of Escherichia coli K-12 is presented. Of 4288 protein-coding genes annotated, 38 percent have no attributed function. Comparison with five other sequenced microbes reveals ubiquitous as well as narrowly distributed gene families; many families of similar genes within E. coli are also evident. The largest family of paralogous proteins contains 80 ABC transporters. The genome as a whole is strikingly organized with respect to the local direction of replication; guanines, oligonucleotides possibly related to replication and recombination, and most genes are so oriented. The genome also contains insertion sequence (IS) elements, phage remnants, and many other patches of unusual composition indicating genome plasticity through horizontal transfer.

Keywords

GenomeGeneBiologyGeneticsEscherichia coliWhole genome sequencingHorizontal gene transferOrigin of replicationComputational biology

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Publication Info

Year: 1997
Type: article
Volume: 277
Issue: 5331
Pages: 1453-1462
Citations: 7392
Access: Closed

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APA Style

                            
                                
                                    Frederick R. Blattner, 
                                
                                    Guy Plunkett, 
                                
                                    Craig A. Bloch
                                
                                et al.
                            
                            (1997). 
                            The Complete Genome Sequence of <i>Escherichia coli</i> K-12. 
                            Science
                            , 277
                            (5331)
                            , 1453-1462.
                            https://doi.org/10.1126/science.277.5331.1453
                        

Identifiers

DOI: 10.1126/science.277.5331.1453

The Complete Genome Sequence of <i>Escherichia coli</i> K-12