Abstract

Experiments in which immobilised restriction fragments of genomic DNA were hybridised with a cloned human fibroblast interferon cDNA indicate that the homologous chromosomal genes exist in only one basic arrangement. This is in marked contrast to recent studies by Nagata et al. (1) showing that there are at least eight gene arrangements for human leukocyte interferon. Having isolated a chromosomal human fibroblast interferon gene from a gene bank, we conclude from nucleotide sequencing studies that there is a complete absence of introns within the RNA-coding region. In view of a similar observation recently made for a human leukocyte interferon gene (1), it would appear as if interferon genes in general are unlike the vast majority of eukaryote genes in this respect.

Keywords

BiologyIntronGeneGeneticsExonInterferonMolecular biology

MeSH Terms

Base SequenceChromosomesHumanCloningMolecularDNA Restriction EnzymesDNARecombinantFibroblastsGenesHumansInterferonsLeukocytesNucleic Acid HybridizationProtein Biosynthesis

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Publication Info

Year
1981
Type
article
Volume
9
Issue
2
Pages
247-266
Citations
85
Access
Closed

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Cite This

Michael Houghton, Ian J. Jackson, Alan G. Porter et al. (1981). The absence of introns within a human fibroblast interferon gene. Nucleic Acids Research , 9 (2) , 247-266. https://doi.org/10.1093/nar/9.2.247

Identifiers

DOI
10.1093/nar/9.2.247
PMID
6163136
PMCID
PMC326690

Data Quality

Data completeness: 86%