Abstract

A thousandfold miniaturization of immobilized optical fiber sensors, a millionfold or more sample reduction, and at least a hundredfold shorter response time, all simultaneously, were achieved by combining nanofabricated optical fiber tips with near-field photopolymerization. Specifically, pH optical fiber sensors were prepared with internal calibration, making use of the differences in both fluorescence and absorption of the acidic and basic dye species. The submicrometer sensors have excellent detection limits, as well as photostability, reversibility, and millisecond response times. Successful applications include intracellular and intraembryonic measurements. Potential applications include spatially and temporally resolved chemical analysis and kinetics inside single biological cells and their substructures.

Keywords

MiniaturizationOptical fiberMillisecondMaterials scienceFiber optic sensorFiberFluorescenceOptoelectronicsNanotechnologyOptics

MeSH Terms

AluminumAnimalsBiosensing TechniquesEmbryoMammalianFiber Optic TechnologyHydrogen-Ion ConcentrationMicroscopyMiniaturizationOptical FibersPhotochemistryPolymersRatsSpectrometryFluorescenceSpectrophotometry

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Publication Info

Year
1992
Type
article
Volume
258
Issue
5083
Pages
778-781
Citations
273
Access
Closed

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Cite This

Weihong Tan, Zhong-You Shi, Steve Smith et al. (1992). Submicrometer Intracellular Chemical Optical Fiber Sensors. Science , 258 (5083) , 778-781. https://doi.org/10.1126/science.1439785

Identifiers

DOI
10.1126/science.1439785
PMID
1439785

Data Quality

Data completeness: 81%